Fig. 2: The increased phagocytosis observed in Tlr4−/− macrophages is partially driven by scavenger receptors. | Nature Communications

Fig. 2: The increased phagocytosis observed in Tlr4−/− macrophages is partially driven by scavenger receptors.

From: Toll-like receptor 4 and macrophage scavenger receptor 1 crosstalk regulates phagocytosis of a fungal pathogen

Fig. 2

a Wildtype iBMDMs (n = 6 per condition), b Tlr4−/− iBMDMs (n = 6 per condition), and d human monocyte derived macrophages (HMDMs) (n = 3 per condition) were treated with oxidised low-density lipoprotein (ox-LDL), a general scavenger receptor ligand, for 30 mins prior to infection with non-opsonised C. neoformans. iBMDM data is pooled from two independent experiments. HMDM data represents two independent experiments with separate donors. c Wildtype and Tlr4−/− iBMDMs (n = 3 per condition) were treated with 10 μg/mL ox-LDL for 30 mins, then infected with C. neoformans opsonised with the anti-capsular 18B7 antibody. The number of internalised fungi per 100 macrophages was quantified from fluorescent microscopy images. Data represents two independent experiments. All data is shown as mean ± SEM; statistical significance was evaluated using an unpaired two-sided t-test (a, b, d); or two-way ANOVA followed by Tukey’s post-hoc test (c). P-values are shown above each graph. Source data are provided as a Source Data file.

Back to article page