Fig. 1: IRX2 expression was increased in fibrotic hearts. | Nature Communications

Fig. 1: IRX2 expression was increased in fibrotic hearts.

From: IRX2 regulates angiotensin II-induced cardiac fibrosis by transcriptionally activating EGR1 in male mice

Fig. 1: IRX2 expression was increased in fibrotic hearts.The alternative text for this image may have been generated using AI.

A Relative mRNA levels of 6 Irx family members in adult mouse cardiac fibroblasts (CFs) after angiotensin II (Ang II) treatment for 24 h (n = 5). B Relative mRNA levels of 6 IRX family members in human CFs after Ang II treatment for 24 h (n = 5). C Irx2 mRNA levels in the heart after Ang II infusion by an osmotic minipump (n = 6). D Representative western blots and statistical analysis of IRX2 expression in the heart after Ang II infusion for 12 weeks (n = 6). E Representative western blots and statistical analysis of IRX2 expression in heart samples obtained from patients with dilated cardiomyopathy (DCM) and control (Con) donors (Con, n = 6; DCM, n = 7). F Irx2 mRNA levels in endothelial cells (ECs), cardiomyocytes (CMs) and CFs isolated from hearts infused with Ang II for 12 weeks (n = 6). G Representative western blots and quantification of IRX2 protein expression in CFs isolated from hearts infused with Ang II for 12 weeks (n = 6). Data are shown as the mean ± SEM, and analysed using an unpaired two-tailed Student′s t test. For the analysis in (C), one-way ANOVA with Tamhane’s T2 test was conducted. Source data are provided as a Source Data file.

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