Fig. 7: Depletion of MSR transcripts in ESCs shifts HP1α condensates into a liquid-like form. | Nature Communications

Fig. 7: Depletion of MSR transcripts in ESCs shifts HP1α condensates into a liquid-like form.

From: Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

Fig. 7

a–f Undifferentiated ESCs treated with an RNA scramble probe (negative control). Two examples of undifferentiated ESCs transfected with an RNA scramble probe, showing fluorescence intensity images (a, d), fluorescence lifetime images (b, e) and histograms of pixel-wise lifetime values (c, f). g–l Undifferentiated ESCs treated with LNA probes. Two examples of undifferentiated ESCs transfected with an LNA probe, showing fluorescence intensity images (g, j), fluorescence lifetime images (h, k) and histograms of pixel-wise lifetime values (i, l). m–r Early-differentiated ESCs treated with LNA probes. Two examples of early-differentiated ESCs transfected with LNA probe, showing fluorescence intensity images (m, p), fluorescence lifetime images (n, q) and histograms of pixel-wise lifetimes values (o, r). A vertical red line shows the 1.44 ns lower boundary of mCherry fluorescence lifetime below which the crowdedness level is above 30% FVO (Fig. 1). Scale bar 2 µm. Fluorescence lifetime colorbar scale from 1.00 and 1.75 ns.

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