Fig. 3: Sequestration of mitochondria in CD81-positive vesicles.
From: Mitochondria are secreted in extracellular vesicles when lysosomal function is impaired
a Co-localization between endogenous CD81 (red) and Cytochrome c (green) in WT +/− Baf A1 (50 nM for 24 h) and Rab7−/− MEFs. Scale bar = 20 μm. Mander’s coefficient (n = 3 independent experiments with a total of 76 cells per condition). b Correlative light and electron microscopy of Rab7−/− MEFs transfected with mPlum-mito3 and CD81-GFP (representative images are from n = 2). Fluorescent image and electron micrograph of a CD81-positive vesicle (green) containing a mitochondrion (magenta) are shown enlarged. c Time-lapse images from live cell imaging of Rab7−/− MEFs overexpressing CD81-GFP (green) and mPlum-mito3 (red) (See Supplementary Movie 1). Arrowheads mark vesicles and mitochondria. Data are mean ± SEM. P values shown are by ANOVA with Tukey’s post-hoc testing. Source data are provided as a Source Data file.
