Fig. 7: TRAIP facilitates resolution of replication transcription encounters. | Nature Communications

Fig. 7: TRAIP facilitates resolution of replication transcription encounters.

From: TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells

Fig. 7: TRAIP facilitates resolution of replication transcription encounters.The alternative text for this image may have been generated using AI.

A PLA assay between TRAIP-mAC (GFP antibodies) and RNA Pol II. Example pictures (top) and quantification of all PLA events per nucleus are presented (bottom). B SPRTN or UBXD7 were downregulated with siRNA in HCT116 TRAIP-mAID cells and the protein level verified by western blot (left). TRAIP-mAID was degraded for 24 h and γH2AX or 53BP1 foci were visualised and quantified in cells in S-phase (right) (n = 3). Mann–Whitney t test; ****p value < 0.0001. Source data are provided as a Source Data file.

Back to article page