Fig. 7: Safety and scope of base editing in tumor modeling.
From: One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids
a Total number of mutations accumulated during either multiplexed or sequential base editor tumor modeling in organoids. Colors in the bars represent mutational signature analysis fit. b Average number of mutations in either multiplexed or sequentially edited organoids. Colors in the bars represent mutational signature analysis fit. n = 3 independent biological samples in case of the multiplexed experiment and n = 4 independent biological samples in case of the sequential experiment. “Data are presented as mean values +/− SD”. c Number of mutations in gene bodies (light) compared to expected (dark) based on their size and total amount of mutations in both SBS2-like (blue) and other (orange) observed mutations. d log2 normalized depletion scores for both SBS2-like and observed mutations that do not correlate with SBS2. Target scope of adenine and cytosine base editors showing the total amount of pathogenic variants in oncogenes (e) and tumor suppressors (f). Pie-charts showing the percentage of oncogenes (g) and tumor suppressors (h) in which at least one pathogenic mutation can be modeled by adenine and cytosine base editors. Asterisks show significance based on binominal testing. Source data are provided as a Source data file.
