Fig. 5: CR1 expression on the RBC surface is correlated to the rs11117991 genotype in both sample cohorts. | Nature Communications

Fig. 5: CR1 expression on the RBC surface is correlated to the rs11117991 genotype in both sample cohorts.

From: Elucidation of the low-expressing erythroid CR1 phenotype by bioinformatic mining of the GATA1-driven blood-group regulome

Fig. 5: CR1 expression on the RBC surface is correlated to the rs11117991 genotype in both sample cohorts.The alt text for this image may have been generated using AI.

Flow cytometry with PE-conjugated anti-CD35 with a the Swedish cohort (n = 100) performed on fresh RBCs grouping by both rs11117991 and rs2274567 genotype, and b a subset of the Thai cohort (n = 41) on thawed RBCs when grouped by rs11117991 genotype, and c grouped by rs2274567 genotype. Geometric mean fluorescence intensities (MFI) were compared when grouped by either the rs11117991 or rs2274567 genotypes. For the Swedish cohort, rs11117991 and rs2274567 genotypes were in perfect concordance and could not be separated. Boxplots indicate median and 1st and 3rd quartiles and error bars indicate values within 1.5 times of the interquartile range; dots outside the bars indicate values greater than 1.5 times the interquartile range. For a–c, the MFI was compared between each genotype group with one-way ANOVA and post-hoc test with the Bonferroni method. d Multiple linear regression analysis of the Thai cohort with geometric MFI as the dependent variable and the genotypes of rs11117991 and rs2274567, as independent variables with ordinary least squares (OLS) linear regression. The coefficients and the 95% confidence intervals (CIs) are plotted against the dependent variable. Variables were assigned according to genotype as follows: the homozygous major was designated as 0, heterozygous as 1, and homozygous minor as 2. No additional adjustments were made for multiple comparisons. P-values: *, <0.05; ***, <0.001. Source data are provided as a Source Data file.

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