Fig. 6: Data-driven analysis of injected inner body proteins.

A ROC curve for virion MS (AUC ≈ 0.94) using as ground truth the prior reported virion proteins. Green highlights the selected threshold for maximum sensitivity at the lowest FPR. B Density plot representing the enrichment of virion proteins over a whole proteome infection experiment expressed as log2 fold change (x-axis). Different colors represent whether a protein was previously reported as a virion (purple), a novel from our virion dataset (green), or a non-virion (cream). C Sequence logo for the proteins in the IB interaction network. The x-axis shows the position from N to C term while the y-axis represents conservation in bits. D SEC-MS derived interaction network for the reported IB proteins (gp93/95/97). Color code represents the query protein (aquamarine), processed virion protein (dark green), non-processed virion proteins (dark purple), previously reported inner body proteins (orange) and proteins not identified in the virion MS experiment (light yellow). E Injection of phage proteins evaluated by western blot of 3× FLAG phage tagged proteins. gp089 (−ve ctrl) serves as a negative control, while gp093 (+ve ctrl) serves as a positive control (n = 1). Validated novel injected proteins are designated with an asterisk.