Fig. 4: Axial-scanning-free imaging of a 3D object. | Nature Communications

Fig. 4: Axial-scanning-free imaging of a 3D object.

From: Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus

Fig. 4

a Sketch of a 3D metallic fishnet wedge composed of an array of circular holes with a period of 2 μm. The depth of the wedge varies from 0.05 μm at the left edge to 1.3 um at the right edge, and the diameter of the holes is about 500 nm. b Top-view (x-y plane) SEM micrograph of a fabricated fishnet wedge. The two insets at the bottom are the zoom-in images of a single hole (left panel) and the top-right cornner of wedge. c–e Images of the wedge in b collected by the wide-field microsocpe c, LSCM d, and our DOF-extended SOL-based microscope all working in the transmission mode (T-mode).

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