Fig. 5: The precise detection of intrauterine foreign body in vivo.

a A typical NIR-IIx + NIR-IIb fluorescence hysterography image. Scale bar, 5 mm. b Line SBR results along the white lines in (a) and Supplementary Fig. 55a. c The signal areas after binarization and the area SBRs of the uterus images in different spectral regions. The statistical analysis is conducted using three segmented images with three TH values (n = 3) and the mean area SBRs are shown in the columns. Error bars indicate SEM. d The FWHMs in different collection spectral regions along the blue lines in (a) and Supplementary Fig. 55a. e The operation illustration for the intrauterine foreign body detection. f The picture of the mouse after operation with the abdomen sutured. g The NIR-IIx + NIR-IIb fluorescence images with the peristalsis and manual pressing and the binary images with the black spots representing the foreign bodies. The white arrows and the green arrows point out the foreign bodies. Scale bar, 10 mm. h The illustration of pregnancy. The fluorescence uterine images of pregnant mouse with i 1300-nm, j 1400-nm and k 1500-nm LP detection. l The merged image of red (1300LP), green (1400LP) and blue (1500LP) channels. m The 1400LP image where the fetuses are marked with white arrows. Scale bar, 10 mm. n The fluorescence images of the missed abortion model before opening the abdomen and the cross-sectional fluorescence intensity profile along the yellow line of the residual pregnancy tissue. a.u. here represents arbitrary units. o The fluorescence images and the cross-sectional fluorescence intensity profile along the yellow line of the fetus. Scale bar, 10 mm. The Gaussian fits to the profiles are shown by the brown lines in (n) and (o). a.u. here represents arbitrary units. p The fluorescence image of the missed abortion model after opening the abdomen. q The pictures of the isolated uteruses. Representative histological images of the r right uterus (containing fetus) and s left uterus (containing residual pregnancy tissue) in three independent experiments. Scale bar in (r), 1 mm; Scale bar in (s), 500 μm.