Fig. 5: Projection refines spatial expression patterns observed in enterocytes of the intestinal villus. | Nature Communications

Fig. 5: Projection refines spatial expression patterns observed in enterocytes of the intestinal villus.

From: Projecting RNA measurements onto single cell atlases to extract cell type-specific expression profiles using scProjection

Fig. 5: Projection refines spatial expression patterns observed in enterocytes of the intestinal villus.The alternative text for this image may have been generated using AI.

a UMAP plot of the single cell atlas (circles) and projected LCM samples (squares) across the zones of the intestinal villus. Single cells are colored based on their zone assignment by Moor et al. b Heatmap visualizing the spatial expression patterns of the top 3000 highly variable genes using the spatial inference approach of Moor et al. on the left and after projecting the LCM samples with scProjection on the right. Three marker genes (rows) are labeled: Ada, Slc2a2 and Reg1. c Schematic of a single intestinal villus, along with the expected dominant expression zone of expression for Ada, Slc2a2 and Reg1. Shown below the villus is the measured expression pattern of Ada, Slc2a2 and Reg1 in the LCM data of the five zones. d Line plots comparing the measured and projected expression of top zonated genes across the intestinal villus.

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