Fig. 2: HCR-FISH labeling uncovers the molecular architecture of central visual areas.
From: Retina-derived signals control pace of neurogenesis in visual brain areas but not circuit assembly
Marker genes label cells in distinct anatomical regions. a, c–f Substack maximum z-projections of registered HCR-FISH stains. From top to bottom (left): a, c Selected thalamic markers: cort, pth2, crhbp, crhb. d, e Selected pretectal markers: foxb1a, zic1, npy, grm2b. f Selected tectal markers: gjd2b, pou4f2. Alongside each stain are UMAPs (same as in Fig. 1), showing the clustering of glutamatergic (middle) and GABAergic cells (right) that express the marker. UMAPs with no clustered expression were omitted. b In yellow, HGn12C:GFP background stain used for registration (scale bar = 50 µm; applies to all images). For all stains, at least three larvae were imaged. Additional stains and anatomical annotations are available in the zebrafish brain atlas31 at mapzebrain.org. g Gene expression look-up matrix for combined plots.
