Fig. 2: C16orf72 is required for tolerance against replication stress.

a Clonogenic survival assay of C16orf72 knock-out cells (c16orf72Δ.2 and c16orf72Δ.3) treated with increasing concentration of methyl methanesulfonate (MMS) for 1 h (left), phleomycin for 1 h (middle) and mitomycin C (MMC) for 24 h (right). PARP1 knock-out cells (parp1Δ) were used as positive control for MMS sensitivity. b Clonogenic survival assay of C16orf72 knock-out cells (c16orf72Δ.2) and complemented cells (c16orf72Δ.2 expressing Flag-C16orf72) treated with increasing concentration of the replication stress-inducing agent, hydroxyurea (HU), for 24 h. c Clonogenic survival assay of C16orf72 knock-out cells (c16orf72Δ.2) and complemented cells (c16orf72Δ.2 + Flag-C16orf72) treated with increasing concentration of the replication stress-inducing agent aphidicolin, for 24 h. d Western blot analysis of C16orf72 protein in whole-cell extract (WCE) and chromatin-enriched fraction of U2OS cells treated with 2 mM hydroxyurea (HU) for 24 h. Extracts were prepared for untreated or HU-treated cells and western blotting performed with the indicated antibodies. Images are representative of 3 biological repeats. For all clonogenic survival assay plots, mean values ± SEM of 3 biological independent experiments are shown. Statistical analysis performed using two-way ANOVA with replication; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Source data are provided as a Source Data file.