Fig. 6: Differences in CD8+ T cell functionality in progressors versus non-progressors. | Nature Communications

Fig. 6: Differences in CD8+ T cell functionality in progressors versus non-progressors.

From: Atezolizumab plus stereotactic ablative radiotherapy for medically inoperable patients with early-stage non-small cell lung cancer: a multi-institutional phase I trial

Fig. 6

A Box and whisker plots demonstrating differences in the frequency of circulating CD8+ T cells between progressors (P, n = 4 patients) and non-progressors (NP, n = 12 patients) at baseline. The panel below depicts representative flow staining for CD4+ T cells and CD8+ T cells on CD3+ gated lymphocytes in NP (left panel) and P (right panel). B ROC curve evaluating the ability to classify P vs. NP based on baseline levels of circulating CD8+ T cells. C Box and whisker plots demonstrating differences in the frequency of circulating CD8+ PD1+ T cells in P vs. NP at baseline (n = 16). D Representative flow staining for PD1 on CD8+ gated T cells in FMO negative control (top), NP (bottom left), and P (bottom right). E ROC curve evaluating the ability to classify P vs. NP based on baseline levels of circulating CD8+ PD1+ T cells. F Box and whisker plots demonstrating differences in the frequency of circulating CD8+Tim3+ T cells in P vs. NP at baseline (n = 16). G Representative flow staining for Tim3 on CD8+ gated T cells in FMO negative control (top), NP (bottom left), and P (bottom right). H ROC curve evaluating the ability to classify P vs. NP based on baseline levels of circulating CD8+ Tim3+ T cells. I Schema of ex-vivo T cell stimulation assay. Box plots and line graphs (left depicting the frequency of interferon-gamma (J, K, N, O) and TNF-α (L, M, P, Q) after ex-vivo PMA/ionomycin stimulation on PD1- (J–M) and PD1+ (N–Q) CD8+ T cells collected at baseline or after 1 cycle of ICI (n = 8 per timepoint; NP, n = 5; P, n = 3). For box and whisker plots, each dot represents the value for an individual patient, the line represents the median, the box represents the interquartile range, and the whiskers represent the spread of the data. For line graphs, dots represent the mean, and error bars represent the standard deviation. Patients free from progression are represented in blue, and patients who progressed are represented in red. P values are from a two-sided t-test, * = p < 0.05. Source data are provided as a Source Data file.

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