Fig. 1: Crystal structure of CarH in dark- and light-adapted states.
From: Redox driven B12-ligand switch drives CarH photoresponse
a Cartoon representation of a full-length dark monomer, showing the different domains and the binding location of AdoCbl (shown as sticks) at the interface of subdomains of CBD (PDB 5C8D). b, c Dark tetramer capable of binding DNA via the DBD (shown in orange for all monomers) (PDB 5C8E). CBD region coloured in green and blue. d Light-adapted monomer obtained following photolysis of the Co-C bond in AdoCbl and tetramer dissociation in solution (PDB 5C8F). e, f Close-up view of the AdoCbl binding site in individual monomers of dark-state (e) and light-adapted (f) CarH12. The residues participating in important interactions are shown as sticks. Cobalamin is shown in beige and the adenosyl moiety is depicted in grey.
