Fig. 5: Depletion of Tnfaip3 in Langerin⁺ cDC1s suppresses type-2 skewing of Tc cells which is restored by blocking IFNγ.

A Schematic overview of AAI model induced by HDM: WT and Tnfaip3^Lg-KO mice were sensitized intranasally (i.n.) with PBS or 1 μg HDM on day 0 and challenged i.n. with 10 μg HDM daily from day 7 to day 11. 0.5 mg anti-IFNγ was given intraperitoneal (i.p.) on days −11, −7, −4, 0, 3, 7, 10, and 14. Analysis was performed on day 15. B Numbers of eosinophils, CD4⁺, and CD8⁺ T cells determined in BAL by flow cytometry analysis. C Quantification of IFNγ⁺, IL-5⁺, and IL-17A⁺ Th and Tc cells in BAL by flow cytometry. D Pie charts summarizing proportions of Tc1, Tc2, and Tc17 cells in PBS or HDM-sensitized WT or Tnfaip3^Lg-KO mice treated with or without anti-IFNγ. ‘% cytokine of total:’ indicates percentage of all Tc cells that produce one of the cytokines assayed. Symbols represent individual mice, B-C: n = 6 mice per group, bars indicate mean values ±SEM. *P < 0.05, **P < 0.01 (two-tailed Mann–Whitney U test). AAI allergic airway inflammation, HDM house dust mite, WT wild type, BAL bronchoalveolar lavage. Source data are provided as a Source Data file.