Fig. 7: Type-2 skewing by IL-33 in the context of an antigen-specific Tc response.

A Schematic overview of eosinophilic airway inflammation induced by IL-33 and/or ovalbumin (OVA): naive OTI cells were first transferred into WT recipient mice. The next day, mice were challenged intratracheally with 50 μg OVA and 10 μg HDM, 0.5 µg IL-33, or a combination treatment. Analysis was performed on day 5. B Numbers of IFNγ⁺, IL-4⁺, IL-5⁺, and IL-13⁺ OTI Tc cells were determined in the mediastinal lymph node (MLN) by flow cytometry. PBS: n = 5, OVA + HDM: n = 6 mice. C Numbers of eosinophils and OTI Tc cells determined in BAL by flow cytometry. D Quantification of IFNγ⁺, IL-4⁺, IL-5⁺, and IL-13⁺ OTI Tc cells in BAL by flow cytometry. Symbols represent individual mice, C, D n = 6 mice per group, bars indicate mean values ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Mann–Whitney or Kruskal–Wallis test corrected for multiple testing). WT wild type, BAL bronchoalveolar lavage. Source data are provided as a Source Data file.