Table 1 Cryo-EM data collection and refinement statistics
From: Hydrophobic interactions dominate the recognition of a KRAS G12V neoantigen
|  | V2-Fab–KRASG12V/HLA-A*03:01 (PDB ID 7STF) |
|---|---|
Data collection | |
EM equipment | Titan Krios |
Voltage (kV) | 300 |
Detector | Gatan K3 |
Energy filter | Gatan Bioquantum |
Pixel size (Ã…) | 0.844 |
Electron dose (e−/Å2) | 60 |
Defocus range (μm) | −0.8 to −2.0 |
Reconstruction | |
Software | RELION 3.1 |
Number of used particles | 116,685 |
Symmetry imposed | C1 |
Overall resolution (Ã…) | 3.14 |
Refinement | |
Software | Phenix |
Cell dimensions | |
 a, b, c (Å) | 70.05, 64.14, 145.17 |
 α, β, γ (°) | 90, 90, 90 |
Model composition | |
 Protein residues | 817 |
R.m.s. deviations | |
 Bonds (Å) | 0.011 |
 Angles (°) | 1.705 |
Ramachandran (%) | |
 Favorable | 81.12 |
 Allowed | 17.76 |
 Outlier | 1.12 |
 Molprobity score | 3.46 |
