Fig. 4: The coupling of MOF and the intrinsic CCM of C. pyrenoidosa.

a The average biomass growth rate of C. pyrenoidosa within two-day cultivation with the addition of 50 ppm MOF, 1 mM AZA (acetazolamide, inhibit external carbonic anhydrase, eCA) and both of them at different pH. b CA activity measurements of the supernatants of microalgal suspensions which cultivated with or without MOF at different pH under air. c The Michaelis constant K_1/2 values as calculated from the Michaelis-Menten fit of the plot of the net O₂ evolution rate versus the concentration of CO₂ for C. pyrenoidosa (Algae) and MOF/C. pyrenoidosa (MOF/Algae) cells grown at different pH under air (LC, 0.04%), error bars in (c) represent the standard deviation of K_1/2 values as fitted from three independent experimental results. Cell density of 1.0 × 10⁷ cell mL^–1 and the light source with a cutoff wavelength filter (under 50 μE m⁻² s⁻¹ irradiation, λ > 600 nm). d The linear fitting curves of the kinetic plots of the reaction that the hydration of CO₂ into HCO₃⁻ in different environments (pH 7.0 20 mM HEPES buffer, the supernatant of microalgal suspension after two-day cultivation, and the addition of bare MOF or MOF pre-treated in supernatant), pH changes indicate the accumulation of products since proton generated simultaneously with HCO₃⁻ in this process. e The apparent activities of the Rubisco based on the dry cell weight of biomass and the total protein, respectively. f The protein, carbohydrate and lipid contents of C. pyrenoidosa cultured two days in the absence (Algae) and presence (MOF/Algae) of MOF. Cultivation conditions: temperature, 26 °C; light intensity, 50 μE m⁻² s⁻¹; 20 mL min⁻¹ ambient air flow (LC); 24 h continuous illumination; 20 mM HEPES buffer (initial pH 7.0). Error bars in a–f represent the standard deviation of the results from three biologically independent experiments. Source data are provided as a Source Data file.