Fig. 4: The binding of NFIB and the Pre-RC on chromatin.
From: NFIB facilitates replication licensing by acting as a genome organizer
a Genome tracks show the signals of nascent strands, chromatin accessibility, NFIB, and ORC1. PRKAR2A and ECSIT are the Refseq gene names. b qChIP verification of chromatin binding of NFIB and pre-RC at the origins shown in A using antibodies against the indicated proteins. The data are presented as the means ± SD for triplicate experiments. P values were determined by an unpaired two-tailed T test. c U2OS cells were synchronized at the G2/M phase and released for different hours. qChIP experiments were performed with antibodies against the indicated proteins to examine the chromatin binding dynamics of NFIB and ORC1. Error bars represent mean ± SD for triplicate experiments. P values were determined by an unpaired two-tailed T test. d U2OS cells were infected with lentiviruses carrying the indicated shRNAs or transfected with the indicated siRNAs. qChIP experiments were performed using antibodies against the indicated proteins. Error bars represent mean ± SD for triplicate experiments (*P < 0.0001, 2-way ANOVA). e Knockdown efficiency of the indicated factors was verified by western blotting.
