Fig. 1: Stable overexpression of pgph-2 decreases fat accumulation, extends healthspan and protects from glucotoxicity. | Nature Communications

Fig. 1: Stable overexpression of pgph-2 decreases fat accumulation, extends healthspan and protects from glucotoxicity.

From: Glycerol 3-phosphate phosphatase/PGPH-2 counters metabolic stress and promotes healthy aging via a glycogen sensing-AMPK-HLH-30-autophagy axis in C. elegans

Fig. 1

For all figure panels, **** represents P < 0.0001. a Oil red O staining and quantification in Control and pgph-2 o/e 1-day adult animals treated with or without 2% glucose. Data represent mean ± SEM, n = 3 independent experiments. Scale bar = 50 µm. P-values are obtained using one-way ANOVA with the Bonferroni test. b, c Bar plots showing pharyngeal pumping rates of control and pgph-2 overexpressing nematodes at indicated days of age at normal growth conditions (b) and 2% glucose conditions (c). Data represent mean ± SEM, n = 3 independent experiments. P-values are obtained by two-way ANOVA with Bonferroni correction. d, e Body bends per second of pgph-2 o/e animals in comparison to controls in normal growth (d) and 2% glucose conditions (e). Data is shown using dot plots with denoted mean ± SEM, n = 3 independent experiments. The number of tracks is shown in datasource. P-values are obtained by two-tailed unpaired Student’s t-test. f, g Lifespan of pgph-2 overexpressing animals in comparison to control nematodes in normal growth conditions (f) and on plates supplemented with 2% glucose (g). The number of separate lifespan experiments, animals and detailed statistics are shown in Supplementary Data 1. P-value is obtained using the two-sided Mantel–Cox test. h Brood size as measured by the number of progeny per animal in eat-2 (ad465); control; eat-2 (ad465), and pgph-2 o/e; eat-2 (ad465) grown on normal growth medium or plates supplemented with 2% glucose. Data are shown as dot plots representing mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni test. i Locomotion analysis on days 9 and 12 of age, measured by body bends per second in indicated strains grown on plates supplemented with 2% glucose. Data represent mean ± SEM, n = 3 independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni test. j Lifespan of control, pgph-2 o/e, pgph-2 o/e; daf-16 (mu86), and daf-16 (mu86) overexpressing animals on plates supplemented with 2% glucose. The number of experiments, animals and detailed statistics are shown in Supplementary Data 1. P-values are obtained using the two-sided Mantel–Cox test.

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