Fig. 4: PGPH-2 o/e activates autophagy in an HLH-30-dependent manner.

For all figure panels, **** represents P < 0.0001. a Representative confocal images and quantification of LGG-1::mCherry puncta in the intestines of day 1 WT and pgph-2 o/e animals under normal growth conditions. The scale bar = 20 µm. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by unpaired two-tailed Student’s t-test. Graphical representation created using Biorender.com. b Representative confocal images and quantification of LGG-1::mCherry puncta in the intestines of day 1 WT and pgph-2 o/e animals grown on plates supplemented with 2% glucose. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni correction. The scale bar = 20 µm. c Representative confocal images and quantification of LGG-1::mCherry puncta in the intestines of day 1 WT, pgph-2 o/e, pgph-2 o/e; hlh-30 (tm1978), and hlh-30 (tm1978) animals grown on normal growth condition or plates supplemented with 2% glucose. The scale bar = 20 µm. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni test. d Locomotion analysis on day 9 of age, measured by body bends per second control, pgph-2 o/e, pgph-2 o/e; atg-18 (gk378), and atg-18 (gk378) strains grown on plates supplemented with 2% glucose. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni correction. Number of tracks is shown in datasource. e Lifespan of control, pgph-2 o/e, pgph-2 o/e; atg-18 (gk378), and atg-18 (gk378) overexpressing animals on plates supplemented with 2% glucose. The number of separate lifespan experiments, animals and detailed statistics are shown in Supplementary Data 1. P-value is obtained using the two-sided Mantel–Cox test.