Fig. 5: PGPH-2 o/e activates the AMPK-HLH-30-autophagy signaling cascade to promote healthy aging under glucotoxicity. | Nature Communications

Fig. 5: PGPH-2 o/e activates the AMPK-HLH-30-autophagy signaling cascade to promote healthy aging under glucotoxicity.

From: Glycerol 3-phosphate phosphatase/PGPH-2 counters metabolic stress and promotes healthy aging via a glycogen sensing-AMPK-HLH-30-autophagy axis in C. elegans

Fig. 5

For all figure panels, **** represents P < 0.0001. a Percent nuclear translocation of HLH-30 in young adult control animals and pgph-2 o/e animals on EV or TOR RNAi conditions. Data represent mean ± SEM from four independent experiments. P-values are obtained by one-way ANOVA with Bonferroni correction. b Brood size as measured by the number of progeny per animal in control and pgph-2 o/e animals grown on Ev plates or TOR RNAi plates. Data represent mean ± SEM from four independent experiments. P-values are obtained by one-way ANOVA with Bonferroni correction. c Western blot analysis and quantification by Image J of pAMPK and Tubulin in control, pgph-2 o/e, and starved control animals. Data represent mean ± SEM from four independent experiments. P-value is obtained by unpaired one-tailed Student’s t-test. d Percent nuclear translocation of HLH-30 in young adult control, pgph-2 o/e, pgph-2 o/e; aak-2 (ok524), and aak-2 (ok524) animals, grown on normal growth conditions or plates supplemented with 2% glucose. Data represent mean ± SEM from more than three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni correction. e, f Representative confocal images (e) and quantification of LGG-1::mCherry puncta (f) in the intestines of day 1 WT, pgph-2 o/e, pgph-2 o/e; aak-2 (ok524), and aak-2 (ok524) animals grown on normal and 2% glucose conditions. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni correction. The scale bar = 20 µm. g Locomotion analysis on days 9 and 12 of age in control, pgph-2 o/e, pgph-2 o/e; aak-2 (ok524), and aak-2 (ok524) strains grown on plates supplemented with 2% glucose. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni correction. h Lifespan of control, pgph-2 o/e, pgph-2 o/e; aak-2 (ok524), and aak-2 (ok524) overexpressing animals on plates supplemented with 2% glucose. The number of separate lifespan experiments, animals and detailed statistics are shown in Supplementary Data 1. P-values are obtained using the two-sided Mantel–Cox test.

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