Fig. 7: PGPH-2 o/e reduces glycogen stores to activate an AMPK-dependent/HLH-30/autophagy cascade. | Nature Communications

Fig. 7: PGPH-2 o/e reduces glycogen stores to activate an AMPK-dependent/HLH-30/autophagy cascade.

From: Glycerol 3-phosphate phosphatase/PGPH-2 counters metabolic stress and promotes healthy aging via a glycogen sensing-AMPK-HLH-30-autophagy axis in C. elegans

Fig. 7

For all figure panels, **** represents P < 0.0001. a, b Representative iodine staining images and quantification in control and pgph-2 o/e animals in normal growth conditions (a) and plates supplemented with 2% glucose (b). The scale bar = 50 µm. Data represent mean ± SEM from three independent repeats. P-values are obtained using two-sided unpaired Student’s t-test (a) and one-way ANOVA with Bonferroni correction (b). The scale bar = 50 µm. c Glycogen degradation pathway. d Representative iodine staining images and quantification in WT and pgph-2 o/e exposed to Ev and pygl-1 RNAi. Data represent mean ± SEM from three independent repeats. P-values are obtained using one-way ANOVA with Bonferroni correction. e HLH-30 nuclear translocation in WT and pgph-2 o/e animals expressing the HLH-30::GFP transgene exposed to Ev and pygl-1 RNAi for two generations and grown on normal conditions or excess glucose conditions. Data represent mean ± SEM from three independent experiments. P-values are obtained using one-way ANOVA with Bonferroni correction. f Representative confocal images and quantification of LGG-1::mCherry puncta in the intestines of day 1 WT and pgph-2 o/e exposed to Ev and pygl-1 RNAi for two generations and grown on normal or excess glucose conditions. Data represent mean ± SEM from three independent experiments. P-values are obtained using one-way ANOVA with Bonferroni correction. The scale bar = 20 μm. g Locomotion analysis on days 9 and 12 of age, measured by body bends per second in control and pgph- 2 o/e exposed to Ev and pygl-1 RNAi bacteria and grown on plates supplemented with 2% glucose. Data is shown using dot plots with denoted mean ± SEM from three independent experiments. P-values are obtained using one-way ANOVA with the Bonferroni correction and exact number of tracks is indicated in datasource. h Lifespan of control and pgph-2 o/e exposed to Ev and pygl-1 RNAi bacteria and grown on plates supplemented with 2% glucose. P-value is obtained using the two-sided Mantel–Cox test.

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