Fig. 3: Thermal stability is influenced by methylation.
From: A qPCR technology for direct quantification of methylation in untreated DNA
a Sequences of the oligonucleotides used for the study. A fluorescent probe was synthesized with a FAM™ fluorophore (F) in the 5’-end and a black hole quencher 1 (BHQ®-1) in the 3’-end. The probe covered a sequence found in the promoter region of MGMT comprising four CpG sites. The probe was hybridized to a complementary oligonucleotide being either unmethylated (UM) or methylated at all four cytosine nucleobases (M). Created with BioRender.com. b Thermal stability of the two duplexes. The unmethylated DNA duplex (red) had a melting temperature (Tm) of 64.6 ± 0.03 °C and the methylated complex (blue) melted at 68.9 ± 0.08 °C giving a difference of 4.3 °C (95% CI [4.2; 4.5]).
