Fig. 5: Performance studies from the EpiDirect® MGMT Methylation qPCR Assay.
From: A qPCR technology for direct quantification of methylation in untreated DNA
a Linearity was observed from 100% to 2.5% methylation. The ΔCt values are plotted as a function of the logarithm to the percentage of methylation of the DNA template. Each sample was evaluated in 10 replicates from multiple PCR runs. b Experimental data for 20 replicates of unmethylated and 3% methylated DNA. The unmethylated DNA was evaluated using DNA purified from whole blood (red) and FFPE material (blue). 3% methylated DNA (green) and 3% methylated DNA diluted in DNA from FFPE material (purple) were evaluated. Nineteen out of twenty replicates were detected for the 3% methylated DNA samples. The technical limit of detection (LOD) is plotted as a broken line at ΔCt = 4.9. Five ng of DNA was used as input for each replicate. Source data are provided as a Source Data file for Fig. 5a and 5b.
