Table 1 Probes and target sequences used for the analysis of DNA and INA® affinities towards unmodified and 5mC modified target sequences
From: A qPCR technology for direct quantification of methylation in untreated DNA
Sequence ID | Name | Sequence 5′ to 3′-end |
|---|---|---|
P1 | Ref | FAM-CGTCCCGACGCCCG-BHQ1 |
P2 | INA-1 | FAM-C1GTCCC1GAC1GCCC1G-BHQ1 |
P3 | INA-2 | FAM-C2GTCCC2GAC2GCCC2G-BHQ1 |
T1 | UM | CGGGCGTCGGGACG |
T2 | M | mCGGGmCGTmCGGGAmCG |
T3 | 1mC | mCGGGCGTCGGGACG |
T4 | 2mC | CGGGmCGTCGGGACG |
T5 | 3mC | CGGGCGTmCGGGACG |
T6 | 4mC | CGGGCGTCGGGAmCG |
T7 | 1,2mC | mCGGGmCGTCGGGACG |
T8 | 1,2,3mC | mCGGGmCGTmCGGGACG |
