Fig. 1: Nuclear and cytoplasmic lncRNA identification.

a A general overview of the lncRNA identification pipeline. Created with BioRender.com. b Validation of cell fractionation efficiency using anti-histone H3 and anti-aldolase as nuclear (Nuc) and cytoplasmic (Cyt) markers. Blot is representative of two independent biological replicates. c Comparison of lncRNA candidates with lncRNAs identified from previous publications. d Essentiality of lncRNAs using piggyBac insertion (Zhang et al., 2018). LncRNAs that cannot be disrupted are more likely to be essential.