Fig. 5: Chromatin Isolation by RNA Purification (ChIRP). | Nature Communications

Fig. 5: Chromatin Isolation by RNA Purification (ChIRP).

From: Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum

Fig. 5

a Schematic representation of the ChIRP methodology. Created with BioRender.com. RBP: RNA-binding protein. b RT-PCR following the ChIRP protocol validates the specificity of the biotinylated antisense probes. Following the ChIRP-pulldown, the RNA fraction was analyzed, and RT-PCR results confirm that lncRNA-TARE-4 probes retrieve the lncRNA-TARE-4 RNA (535 bp PCR product) and the control serine tRNA ligase probes retrieve the serine tRNA ligase RNA (505 bp PCR product), respectively. No RNA was retrieved in the no probe control. RNA from a ChIRP-input sample as well as WT 3D7 parasites (wells 4 and 5, respectively) was used to confirm the lncRNA-TARE-4 and serine tRNA ligase primers. The negative controls (well 6) represent no template controls. RT-PCRs are representative of two independent replicates.

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