Fig. 4: Primary PDAC subtypes show distinct localization within the epithelia.
From: Coordinated single-cell tumor microenvironment dynamics reinforce pancreatic cancer subtype
a UMAP projection of the integrated epithelium of both normal and PDAC-derived samples (left). Splitting this subset by source shows distinct subpopulation grouping (right). b Density plots show general localization of common PDAC-related markers (EPCAM: Epithelium, MUC1: Malignancy, AMBP: Normal-like, PRSS1: Acinar, KRT17: Basal-like, TFF3: Classical). c Expression heatmap shows the average epithelial expression (pseudobulk) for each patient (column) using the top 25 genes for both basal and classical signatures (rows). Column tracks show the associated clinical metadata and consensus clustering subtype results (blue: classical, orange: basal, green: mixed). d UMAP of PDAC-derived epithelia projected onto a 3D space models the separation and gradation of the transforming cells. e Localization of the basal-like and classical signatures scores highlight distinct regions and juxtaposition with normal duct and acinar cells. f Differential genes (MMP7, SPP1, MUC6) present as markers for inflammation and early precursor cells in the metaplastic subpopulation. g Scatterplot of the subtype scores for each single-cell in the neoplastic subset. Cells are colored by the originating patient’s subtype designation which shows distinct basal and classical groups with an additional density in between (opacity 25%). Source data are provided as a Source Data file.
