Fig. 2: On-phage enzymatic trimming of sialic acid using neuraminidase. | Nature Communications

Fig. 2: On-phage enzymatic trimming of sialic acid using neuraminidase.

From: Chemoenzymatic synthesis of genetically-encoded multivalent liquid N-glycan arrays

Fig. 2

a Preparation and attachment of azido-modified Asn-linked biantennary SGP 7 to M13 phage. b neuraminidase treatment to afford mixtures of conjugates with either one or two terminal galactose residues on pVIII. c MALDI-TOF MS characterization of conjugation of DBCO-NHS to pVIII. d S1 and S2 products of ligation of 7, and S2’, a MALDI-TOF MS artifact. e P1 and P2 phage-displayed glycans generated by neuraminidase treatment.

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