Fig. 8: Synthesis of AdoCbl in vitro.
From: A synthetic cell-free 36-enzyme reaction system for vitamin B12 production
a Titer and productivity of the cell-free reaction system from 5-ALA to AdoCbl during optimization. Reactions were performed in triplicate (n = 3 biologically independent samples) and data are presented as mean values ± SD. b Titer and productivity of the cell-free reaction system from purified HBA to AdoCbl during optimization. The significant strategy causing the improvement of every reaction system was highlight in the right axis of evolution, and specific reaction composition were listed in supplementary Table 2. Reactions were performed in triplicate (n = 3 biologically independent samples) and data are presented as mean values ± SD. Source data are provided as a Source Data file.
