Fig. 2: Attenuated SREBP signaling in LKO livers and primary hepatocytes.
From: Hepatic SREBP signaling requires SPRING to govern systemic lipid metabolism in mice and humans
a Scheme depicting proteomic strategy used to identify and label-free quantify (LFQ) differentially abundant proteins in LKO mice. b Livers were collected from control and LKO male mice (n = 5/group) and subjected to unbiased proteomics analysis (4 h fasted). Volcano plot depicting significantly modulated proteins in control vs. LKO liver with indicated SREBP1 (blue) and SREBP2 (red) regulated targets highlighted. c Pathway analysis shows the top 5 down- and up-regulated canonical pathways in livers from LKO vs. control mice. d Mice were treated as described in Fig. 1e and liver lysates from refed mice were immunoblotted as indicated (n = 4 mice/group). e, f Primary hepatocytes were isolated from control and LKO mice and (e) expression of the indicated genes was determined by qPCR (n = 5 independent mice/group), or (f) immunoblotted as indicated (n = 4 independent mice/group). p-value: Spring (0.0098); Hmgcr (0.0186); Sqle (0.0098) Scd1 (0.034). e Each bar and error represent the mean ± SEM. *p < 0.05, **p < 0.01 analyzed by (e) two-way ANOVA with Holm-Sidak post hoc analysis. Source data are provided as a Source Data file.
