Fig. 1: CCR9 expression is lower in CD4⁺CD8αα⁺ IELs than in other IEL populations.

a Histograms show the CCR9 level among TCRβ⁺CD4⁺CD8α⁺CD8β⁻ Foxp3⁻ (CD4⁺CD8αα⁺; red line), TCRβ⁺CD4⁺CD8α⁻CD8β⁻Foxp3⁻ (CD4⁺CD8αα⁻; green line), and TCRβ⁺CD4⁺CD8α⁻CD8β⁻ Foxp3⁺ (Treg cells; blue line) of IELs and LPLs in the jejunum and ileum. Fluorescence minus one (FMO) control is shown as black line. b Graphs show the mean fluorescence intensity (MFI) of CCR9 among CD4⁺CD8αα⁺, CD4⁺CD8αα⁻, Treg cells, and FMO control of IELs and LPLs in the jejunum and ileum (n = 4 C57BL/6J mice for IEL analysis, n = 5 C57BL/6J mice for LPL analysis, 10 weeks old). c Left; pseudocolor plot shows three populations (ThPOK^lowRunx3^hi, ThPOK^hiRunx3^hi, and ThPOK^hiRunx3^low) among CD4⁺ SI IELs according to the expression of ThPOK and Runx3. Right; representative histogram shows the expression of CCR9 among ThPOK^lowRunx3^hi (red line), ThPOK^hi Runx3^hi (green line), and ThPOK^hiRunx3^low (blue line) CD4⁺ SI IELs. FMO control is shown as black line. d Graph shows the MFI of CCR9 among ThPOK^lowRunx3^hi, ThPOK^hiRunx3^hi, ThPOK^hiRunx3^low and FMO control of CD4⁺ SI IELs (n = 4 Thpok^GFP:Runx3^tdTomato reporter mice, 10 weeks old). e Graphs show relative expression of Zbtb7b, Runx3, Tbx21, and Ccr9 in ThPOK^lowRunx3^hi, ThPOK^hiRunx3^hi, and ThPOK^hiRunx3^low populations among CD4⁺ SI IELs sorted from Thpok^GFP:Runx3^tdTomato reporter mice. Quantitative real-time PCR experiments were performed in duplicate in each sample, and each dots represented as average of duplicate (n = 3). Data are presented as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons post hoc test was applied. Source data are provided as a Source data file.