Fig. 5: Cbfb2 expression is upregulated in CD4⁺CD8αα⁺ IELs of Ccr9^−/− mice.

a Graphs show the relative expression of Runx3, Tbx21, Cbfb1, and Cbfb2 in splenic naive CD4⁺ T cells and in CD4⁺CD8αα⁻ and CD4⁺CD8αα⁺ IELs from Ccr9^+/+ and Ccr9^−/− mice. Quantitative real-time PCR experiments were performed in duplicate in each sample, and each dots represented as average of duplicate (n = 6). b Graphs show the relative expression of Cbfb1 and Cbfb2 in ThPOK^hiRunx3^low, Runx3^hiThPOK^hi, and ThPOK^lowRunx3^hi populations among CD4⁺ SI IELs sorted from Thpok^GFP:Runx3^tdTomato reporter mice. Quantitative real-time PCR experiments were performed in duplicate in each sample, and each dots represented as average of duplicate (n = 3). c Left: Structure of a mouse Cbfb locus that produces two splice variants by distinct splice donor signals within exon 5. cDNA encoding tdTomato or Venus was targeted into exon 6 in the Cbfb locus to monitor Cbfb1 and Cbfb2 splicing by expression of tdTomato and Venus fluorescent protein, respectively. Right: histograms show Cbfb2-Venus and Cbfb1-tdTomato expression in CD4⁺CD8αα⁻ IELs (dotted line) and CD4⁺CD8αα⁺ IELs (red line) from Cbfb2^Venus and Cbfb1^tdTomato reporter alleles, as well as CD4⁺CD8αα⁺ IELs (black line) from wild-type control mice. Data are expressed as mean ± SD. The two-sided Student’s t test (a) or one-way ANOVA with Tukey’s multiple comparisons post hoc test (b) was applied. Source data are provided as a Source data file.