Fig. 3: A3H-Vif interface.
From: Structural basis of HIV-1 Vif-mediated E3 ligase targeting of host APOBEC3H
a Structure of the A3H-Vif subregion in the A3H-VCBCC complex. b Close-up view of the interface between A3H α3 and Vif. c Close-up view of the interface between A3H α4 and Vif. d Interface between A3H loops 4 and 6 and Vif. e Schematic of the amino acid residues involved in the A3H-Vif interactions. f, g Vif-mediated degradation assay of A3H mutants. Mutations were introduced in the Vif-interface residues around α3 and α4 (f) and around loops 4 and 6 (g) on top of K97Q Vif-sensitivity enhancing mutation. The A3H levels in the presence and absence of Vif (+N48H) were probed by Western blots. h Vif-mediated degradation assay using Vif mutants. Mutations were introduced in the A3H-interface residues on top of N48H. A3H levels in the presence of Vif mutants were probed by Western blots. Quantified A3H levels are shown in the right chart. Assays were performed independently in triplicate (mean ± s.d.; n = 3). Source data are provided as a Source Data file.
