Fig. 5: Genetic impact of Myd88 downregulation in Pax5+/− infection-driven leukemia.
a GSEA showing that leukemic Pax5+/−;Myd88+/− cells from diseased mice present similar profiles for genesets previously identified in human B-ALL samples28,29,31. The genesets shown are significantly enriched with a nominal p-value < 1%. Statistical analysis was done using Gene set enrichment analysis (GSEA) algorithm. b Whole Genome Sequencing in Pax5+/−;Myd88+/− B-ALLs. Oncoprint of somatic single nucleotide mutations and copy number alterations across 8 leukemia samples from Pax5+/−;Myd88+/− mice. Somatic alterations are clustered by gene. Tumor DNA was derived from whole leukemic BM or LN, while tail DNA of the respective mouse was used as reference germline material. Previously reported known human or mouse leukemia hotspot mutations are highlighted (red). Mean tumor variant allele fraction (VAF) for each single nucleotide mutation is shown on the dotplot on the right. c GSEA plots showing the 8 gene sets that are significantly enriched at nominal p-value < 1% in Pax5+/−;Myd88+/− proB cells from healthy mice. Enrichment analysis was done using the hallmarks collection from MSigDB55. Statistical analysis was done using Gene set enrichment analysis (GSEA) algorithm.
