Fig. 4: MommeD43 results in improved silencing of DUX4 in a mouse model of FSHD. | Nature Communications

Fig. 4: MommeD43 results in improved silencing of DUX4 in a mouse model of FSHD.

From: SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease

Fig. 4: MommeD43 results in improved silencing of DUX4 in a mouse model of FSHD.

a Diagram of D4Z4 repeat in healthy humans, FSHD1 patients, and the 2.5-unit transgene repeat used in the murine model (described in ref. 44). b–d Relative DUX4, Wfdc3, and Smchd1 transcript levels in myoblasts, myotubes, cerebellum, spleen, and fibroblasts. Bars represent the average transcript levels per genotype (average value in D4Z4-2.5 tissue is set as 1); each dot represents a single mouse, n = 5 per genotype. Statistical analysis was performed using a Student’s t test. *P < 0.05; **P < 0.01, two-tailed. e H3K4me2 levels, H3K9me3 levels, H3K27me3 levels, and the chromatin compaction score (H3K9me3 level corrected for H3K4me2 level) in fibroblast cultures (black dots) and spleen (gray dots). Bars represent the average levels per genotype; each dot represents a single mouse, n = 3 per genotype per tissue/cell type. Statistical analysis was performed using a Student’s t test. *P < 0.05, two-tailed. MommeD43 abbreviated to MD43.

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