Fig. 6: Bafilomycin A1 (BafA) treatment causes a relocalization of GLG1 and TGOLN2 away from the Golgi. | Nature Communications

Fig. 6: Bafilomycin A1 (BafA) treatment causes a relocalization of GLG1 and TGOLN2 away from the Golgi.

From: Deep and fast label-free Dynamic Organellar Mapping

Fig. 6

Widefield imaging of immunofluorescence labelling of GLG1, TGOLN2 (TGN46), and GALNT2 validated the localization shift predictions shown in Fig. 5. HeLa cells were cultured for 1 h in either: (1) full growth medium (Control); (2) EBSS to starve the cells (Starve); (3) full growth medium plus 100 nM BafA (Control + BafA); or (4) EBSS plus 100 nM BafA (Starve + BafA). a GLG1 (green) completely disperses from the Golgi in BafA treated cells, with or without starvation; b TGOLN2 (green) shifts away from the Golgi, with or without starvation; a, b GALNT2 (red) remains unchanged. In the merged images, DAPI labelling of the nucleus is also shown (blue). Scale bars: 10 μm. Note, the relocalization effects are caused by BafA treatment and not by starvation. Images are representative of at least 13 images per condition, from two separate imaging experiments. Brightness levels for display were adjusted individually for each condition, to avoid saturation and aid visualization of signal distribution. Raw data have been uploaded to https://zenodo.org/record/8197844.

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