Fig. 5: MIPergic LNs are differentially activated by different odors.

a Odor-evoked responses of MIPergic LN neurites within several AL glomeruli (far left column). Scale bar = 10 µm. Odors tested were presented at 10⁻² and include: apple cider vinegar (ACV), benzaldehyde, 1-octen-3-ol, 1-hexanol, geranyl acetate, and ammonium hydroxide. For ACV, n = 3 (VM2), 4 (DP1l), 5 (DM4 & VA2), 7 (DM2), 8 (DM5) and 10 animals (DM1 & DP1m). For benzaldehyde, n = 3 (DM2, DM5, DP1l, & VM2), 4 (DM4, DP1m, & VA2), and 5 animals (DM1). For 1-octen-3-ol, n = 3 (DM2, DM5, DP1l, & VM2), 4 (DM4, DP1m, & VA2), and 5 animals (DM1). For 1-hexanol, n = 3 (DM2 & DM5), 4 (DP1l & VM2), 5 (DM4, DP1m, & VA2), and 6 animals (DM1). For geranyl acetate, n = 3 (DM2 & DM5), 5 (DM4, DP1l, VA2, & VM2), and 6 animals (DM1 & DP1m). For ammonium hydroxide, n = 4 (DM2, DM5, & VM2), 5 (DM4, DP1l, & VA2), and 6 animals (DM1 & DP1m). Data are presented as the mean (black line) ± SEM (gray shaded area). Vertical and horizontal scale bars = 0.1 ∆F/F & 1 s (respectively). Odor onset is indicated by the vertical lines running up each column of traces. b Peak response (∆F/F) of MIPergic LN intraglomerular neurites from odor onset to ~1 s after stimulus onset across all glomeruli tested for each stimulus. c Area under the ∆F/F curve (AUC) of MIPergic LN intraglomerular neurites across glomeruli for each stimulus. In all cases: Boxplots display the minimum, 25^th-percentile, median, 75^th-percentile, and maximum of the given data. Glomerular schematics were derived from an in vivo AL atlas¹⁶⁴. Source data are provided as a Source Data file.