Fig. 2: Wide-field calcium imaging reveals IC-DBS modulation of the entire dorsal cortex, with an emphasis on the FC.
From: Unraveling the mechanisms of deep-brain stimulation of the internal capsule in a mouse model
a Schematic of wide-field fluorescence microscopy setup. DBS electrodes were implanted, inserted at an angle, contralaterally to the imaged hemisphere, and targeted the ipsilateral IC of Thy1-GCaMP6f mice (n = 5). b A video frame of the dorsal cortex (left) and the cortical regions (FC frontal cortex, RSP retrosplenial cortex, SS somatosensory cortex, VIS visual cortex) as defined by the Allen Mouse Brain Connectivity Atlas (right)51. c Significant dose-dependent cortex-wide suppression was observed in the varying current (n = 5) and pulse-width (n = 5) experiments, but not in the frequency (n = 5) experiment. d The dose-response manipulations of DBS induced region-specific reduction in FC (current and pulse width) and SS (pulse width), but not RSP nor VIS. e Directly comparing sustained suppression at high-intensity DBS across dorsal-cortical regions revealed significant effects during current and pulse-width, but not frequency, experiments. Post-hoc analyses revealed differences between FC and RPS, and FC and VIS during current manipulations. During pulse-width manipulations, FC differed from VIS. Data are mean ± SEM. Source data are provided as a Source Data file. *p < 0.05, NS not significant.
