Fig. 5: IC-DBS dose-dependently recruits partially overlapping sustained neuron populations, while maintaining excitation/inhibition balance.
From: Unraveling the mechanisms of deep-brain stimulation of the internal capsule in a mouse model
a Anatomical distribution of imaged neurons revealed by maximum-intensity projection of neurons recruited during 100 µA, 200 µA, 300 µA, and overlay thereof (example animal). b In all recorded regions, we found dose-dependent recruitment of sustained neurons in SAPAP3−/− and WT. c DBS recruited significantly more neurons in mOFC of SAPAP3−/− compared to WT across all DBS-parameter experiments (arrow). d Venn-diagram depicts overlap of neuron populations recruited across different current intensities (example region) (left). True percentage of overlapping neuron populations (black horizontal lines) for the different current blocks compared to chance (95% confidence intervals, bootstrap) indicated recruitment of similar neuron populations during the 200 and 300 µA blocks. e For both SAPAP3−/− and WT, all cortical and striatal regions showed significant overlap in DBS-recruited neuron populations for the 200 and 300 µA blocks (arrows). f Maximum-intensity projection of sustained neurons recruited as excited (yellow) or inhibited (blue) during 100 µA, 200 µA, 300 µA, and overlay thereof (example animal). g For both SAPAP3−/− and WT, we found an increased number of excited neurons in mOFC and DS during DBS. In all other regions, the number of excited and inhibited neurons were balanced during DBS (SAPAP3−/−: lOFC (n = 6), mOFC (n = 5), PL (n = 5), M2 (n = 5), DS (n = 4), VS (n = 5); WT: lOFC (n = 5), mOFC (n = 5), PL (n = 5), M2 (n = 4), DS (n = 5), VS (n = 4). Data are mean ± SEM. Source data are provided as a Source Data file. CI=confidence interval, *p < 0.05, NS not significant.
