Fig. 2: In vivo functional analysis of AlpH.
From: O-methyltransferase-like enzyme catalyzed diazo installation in polyketide biosynthesis
a HPLC profiles of the crude extracts from different strains: (i) ΔalpH strain; (ii) ΔalpH::alpH strain; (iii) ΔalpH strain fed with gluN2H3; (iv) Δalp2F2G strain; (v) Δalp2F2G strain fed with gluN2H3; (vi) wild-type strain; (vii) kinamycin D standard; (viii) stealthin C standard. b HPLC profiles of the crude extracts from the E. coli BL21(DE3) derivatives fed with gluN2H3: (i) DHR producing strain plus alpJ-K (Bjk); (ii) Bjk fed with gluN2H3; (iii) DHR producing strain plus alpJ-K-H (Bjkh); (iv) Bjkh fed with gluN2H3; (v) prekinamycin standard; (vi) DHR standard.
