Fig. 5: EPAC1 condensates regulate the transcription of several genes including the large Histone cluster 1.
From: Cyclic AMP induces reversible EPAC1 condensates that regulate histone transcription
a Schematic representation of the experimental design used for the RNAseq experiments (created using Microsoft PowerPoint). b Heatmap of differentially expressed genes (DEGs) between EPAC1-YFP-expressing HEK cells treated with DMSO or the EPAC1-specific activator 8CPT-cAMP (5 µM) for 40′ (p < 0,01). Significance was calculated using Cuffdiff’s t-test analogical methods56. Upregulated genes are represented in red while downregulated ones are in blue. Detail: histone genes differentially expressed. c Heatmap of DEGs between EPAC1Δ2-148-YFP-expressing HEK cells treated with DMSO or 8CPT-cAMP (5 µM). d Percentage of differentially expressed histone genes in the two comparisons. RNAseq experiments were repeated three times independently, for analysis two experiments were used for EPAC1-YFP and three for EPAC1Δ2-148-YFP. Source data are provided (see “Data availability” section).
