Fig. 6: EPAC1 condensates localize at the Histone cluster 1 on chromosome 6.
From: Cyclic AMP induces reversible EPAC1 condensates that regulate histone transcription
a Confocal photomicrographs of colocalization experiments between EPAC1-YFP fluorescence (green) and the chromosome 6 p22.2 locus labeled by a custom-made probe (red) using fluorescence in situ hybridization (FISH) in HEK cells treated with FSK-IBMX. Significant overlap between the DNA probe and EPAC1-YFP was observed in around 40% of the labeled Chr 6p22.2 loci. b, c, d Confocal photomicrographs of EPAC1-YFP (green) and, respectively, (red) chromosome 21 (21q22.13-q22.2), chromosome 12 centromere (CEP) and Chromosome 15 centromere (CEP) loci labeled in FISH experiments using commercial probes, in HEK cells treated with FSK-IBMX. No significant correlation between these sites and EPAC1-YFP (green) was observed. Scale bar, 10 μm (enlargement 1 μm). e Correlation analysis between red and green signals. Lower panel shows colorimetric maps using nMDP values. (Right panel) Graph depicting the calculated index of correlation (Icorr) which represents the fraction of positively correlated (colocalized) pixels (FISH foci analyzed Chr6p22.2 n = 110 over 4 independent experiments; Chr21q n = 34 over 3 independent experiments; CEP15 n = 37 over 3 independent experiments; CEP12 n = 10 over 2 independent experiments). Statistical significance was determined by one-way ANOVA (****p < 0.0001). Error bars: SD. Source data are provided as a Source data file. Nuclei were labeled with DAPI. [FSK] 20 μΜ, [IBMX] 400 μΜ. Scale bar, 1 μm.
