Fig. 1: THBS1 expression is primarily localized in the non-epithelial stroma areas of human CRCs and associated with the aggressiveness. | Nature Communications

Fig. 1: THBS1 expression is primarily localized in the non-epithelial stroma areas of human CRCs and associated with the aggressiveness.

From: THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer

Fig. 1

a, b Representative images of immunostaining (a, n = 3 samples per indicated lesion) and RNAscope (b, n = 3 samples per indicated lesion) of THBS1 in human CRCs and liver metastasis. The experiments were independently repeated three times, yielding similar results. c, THBS1 expression in tumor epithelium (n = 13), and tumor stroma (n = 13) of CRC dataset (GSE35602). d THBS1 expression in each subtype of CMS (top) and IMF (bottom) in TCGA. e Gene set enrichment analysis (GSEA) of the indicated gene sets. f Kaplan-Meier curve in CRC dataset (GSE14333). g–j Staining g in TMAs of human CRCs (THBS1: low n = 102, intermediate n = 122, high n = 107; the rest: low n = 46, intermediate n = 64, high n = 54). Proportion h of CMS4 subtype in indicated groups. Proportion i of CRCs with low-, intermediate- or high-intensity of THBS1 in CMS4-annotated CRCs. Quantification j of CD14 staining in g. k, l Proportion of stage 2 or 3 k and lymph node metastasis l in CRCs with low-, intermediate-, or high-intensity of THBS1 in TMAs. m, n Kaplan-Meier curves of patients corresponding to TMAs. Hazard ratio with 95% confidence interval and P values, analyzed by Log-rank test, are shown in f, m, n. Mean ± SEM. Scale bars, 50 μm a, b, top panels 200 μm and bottom panels 50 μm in each indicated protein g. P values were calculated by two tailed Mann-Whitney test in c, d or two-tailed, unpaired Student’s t test in j. Source data are provided as a Source Data file.

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