Fig. 7: MKP1-p38 MAPK signaling is required for AMPKα-mediated caspase 6 phosphorylation. | Nature Communications

Fig. 7: MKP1-p38 MAPK signaling is required for AMPKα-mediated caspase 6 phosphorylation.

From: MKP1 promotes nonalcoholic steatohepatitis by suppressing AMPK activity through LKB1 nuclear retention

Fig. 7

HepG2 cells were transfected with scrambled siRNA (Scr) or MKP1 siRNA for 24 h followed by 24 h starvation in serum-free medium. Cells were treated with CD medium in the presence of DMSO, 10 μM SB203580 or 10 μM SP600125 for 4 h (for detecting MAPKs and AMPK pathway in a and g) or 24 h (for detecting phosphorylation of caspase 6 in e and k). a Cells were treated with SB203580. Immunoblots of phospho-MAPKAPK2 (Thr334) and HSP90 as a loading control or phospho-p38 MAPK, phospho-AMPKα (Thr172), phospho-AMPKβ1 (Ser182) with the indicated corresponding totals. b–d Quantitation of immunoblots from (a). e Immunoblots of phospho-caspase 6 (Ser257) and caspase 6. f Quantitation of immunoblots from (e). g Cells were treated with SP600125. Immunoblots of phospho-JNK, phospho-AMPKα (Thr172), phospho-AMPKβ1 (Ser182) with the indicated corresponding totals. h–j Quantitation of immunoblots from (g). k Immunoblots of phospho-Caspase 6 (Ser257) and Caspase 6. l Quantitation of immunoblots from (k). Key: CD choline-deficient medium. Data represent the mean ± SEM from 3 independent experiments. p-values were determined by two-way ANOVA.

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