Fig. 2: SAXS analyses and 2-aminopurine (2AP) lifetime probing of the T-box RNA.
From: Direct observation of tRNA-chaperoned folding of a dynamic mRNA ensemble
a, b Overlay of back-calculated SAXS scattering curves (red dotted lines) computed from the cocrystal structure of the N. farcinica ileS T-box riboswitch in complex with tRNAIle (a) or the T-box portion extracted from the complex structure (b) using CRYSOL, with experimental SAXS profiles (black dotted lines). Residuals and χ2 of the fits are indicated. c Pair-wise distance distribution function (PDDF) plots for the tRNA (green), apo T-box (blue), and T-box-tRNA complex (black). d Location of the 2AP probes (A16, orange; A19, red) in the co-crystal structure at the T-box Specifier-tRNA anticodon interface. e, f Time-resolved fluorescence decay time traces for 2AP at positions A16 (e) or A19 (f). Amplitude-weighted averaged lifetimes τavg are indicated. IRF: instrument response function. g Individual lifetimes and relative amplitudes derived from (e, f). The amplitudes for the short, intermediate, and long lifetimes are shown in light blue, green, and red, respectively. Source data are provided as a Source Data file.
