Fig. 6: Solution conformation of ΔΨ T-box RNA.
From: Direct observation of tRNA-chaperoned folding of a dynamic mRNA ensemble
a Cartoon schematic (left) and structural models (right) of the ΔΨ T-box RNA rigid-body docked into the SAXS-reconstructed envelope generated by DAMMIF (gray), in stems I-II docked (upper panel) and stacked (lower panel) conformations. b Overlay of back-calculated SAXS scattering curves (red lines) computed using CRYSOL from the stems I-II docked structures extracted from the cocrystal structure (upper panel) or from the proposed stems I-II stacked structural model (lower panel) with experimental scattering profiles in solution (black lines). Residuals and χ2 of the fits are indicated. c PDDF plots for the apo T-box (gray) and ΔΨ T-box (black). d Effects of inserting a flexible polyuridine linker of different lengths between stems I and II of ΔΨ T-box on tRNA binding affinity. The values are mean ± S.D. from N biologically independent replicates. N = 3 for linker lengths 1 and 10; N = 2 for linker lengths 2–5. * value for zero U-linker length from ref. 10 for comparison. Source data are provided as a Source Data file.
