Table 1 Kinetic parameters of T-box–tRNA bindinga

From: Direct observation of tRNA-chaperoned folding of a dynamic mRNA ensemble

Photobleaching of acceptor 2

Not included

Included

kU12 (h−1)

1.47 (0.34)

kU23 (h−1)

3.55 (0.87)

pU1

0.354 (0.013)

pU2

0.871 (0.011)

kB12 (h−1)

46.8 (2.0)

40.0 (1.7)

kB23 (h−1)

113.4 (4.2)

69.8 (1.7)

kB1 (h−1)

23.4 (0.9)

23.2 (0.9)

kB2 (h−1)

18.0 (0.6)

18.1 (0.4)

kB3 (h−1)

14.5 (0.5)

11.0 (0.3)

ka1 (nM−1h−1)b

1.17 (0.05)

1.16 (0.05)

ka2 (nM−1h−1)b

0.900 (0.030)

0.907 (0.022)

ka3 (nM−1h−1)b

0.725 (0.024)

0.551 (0.014)

kd1 (h−1)

52.6 (1.7)

50.0 (1.4)

kd2 (h−1)

77.4 (2.3)

73.8 (1.6)

kd3 (h−1)

3.08 (0.088)

2.46 (0.052)

φ

0.807 (0.014)

0.814 (0.016)

kbl (h−1)c

 

1.83 (0.65)

  1. aErrors in the parentheses are standard deviations calculated from the curvature at the maximum of the likelihood function.
  2. bkai = kBi/[tRNA], where [tRNA] = 20 nM.
  3. ckbl is the photobleaching rate of acceptor 2.